Crystal structure and kinetic mechanism of aminoglycoside phosphotransferase-2″-IVa
نویسندگان
چکیده
منابع مشابه
Aminoglycoside binding and catalysis specificity of aminoglycoside 2″-phosphotransferase IVa: A thermodynamic, structural and kinetic study
BACKGROUND Aminoglycoside O-phosphotransferases make up a large class of bacterial enzymes that is widely distributed among pathogens and confer a high resistance to several clinically used aminoglycoside antibiotics. Aminoglycoside 2″-phosphotransferase IVa, APH(2″)-IVa, is an important member of this class, but there is little information on the thermodynamics of aminoglycoside binding and on...
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The expression of the bifunctional aminoglycoside inactivating enzyme 6'-N-aminoglycoside acetyltransferase-2"-O-aminoglycoside phosphotransferase is the most important mechanism of high-level aminoglycoside resistance in Staphylococcus and Enterococcus. The enzyme is unique because it presents two different aminoglycoside-modifying activities located in different regions of the molecule. The g...
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Aminoglycoside 2″-phosphotransferases are the major aminoglycoside-modifying enzymes in clinical isolates of enterococci and staphylococci. We describe a novel aminoglycoside 2″-phosphotransferase from the Gram-negative pathogen Campylobacter jejuni, which shares 78% amino acid sequence identity with the APH(2″)-Ia domain of the bifunctional aminoglycoside-modifying enzyme aminoglycoside (6') a...
متن کاملThe crystal structures of substrate and nucleotide complexes of Enterococcus faecium aminoglycoside-2''-phosphotransferase-IIa [APH(2'')-IIa] provide insights into substrate selectivity in the APH(2'') subfamily.
Aminoglycoside-2''-phosphotransferase-IIa [APH(2'')-IIa] is one of a number of homologous bacterial enzymes responsible for the deactivation of the aminoglycoside family of antibiotics and is thus a major component in bacterial resistance to these compounds. APH(2'')-IIa produces resistance to several clinically important aminoglycosides (including kanamycin and gentamicin) in both gram-positiv...
متن کاملSolid–Solid Synthesis, Crystal Structure and Thermal Decomposition of Copper(II) Complex of 2-Picolinic Acid
The copper(II) complex [Cu(pic)2]·2H2O was synthesized with 2-picolinic acid (Hpic) and copper acetate as reactants by room temperature solid-solid reaction. The composition and structure of the complex was characterized by elemental analyses, single crystal X-ray diffraction, X-ray powder diffraction, FT-IR spectroscopy and thermogravimetry-differential scanning calor...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2010
ISSN: 0961-8368
DOI: 10.1002/pro.437